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goat anti-ha-tag polyclonal antibody a00168-40 (GenScript corporation)

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GenScript corporation goat anti-ha-tag polyclonal antibody a00168-40

SLD5 interacts with matrix proteins of several RNA viruses. (a) The nuclear location sequences of M proteins of different RNA viruses were listed. Critical amino acids contributing to a NLS motif are coloured red. (b) Interaction between SLD5 and M proteins by the yeast two-hybrid assay. SLD5 was fused to the pGADT7 vector, M from ICV, MeV, SeV, VSV, ZEBOV, HIV and EIAV were fused to the pGBKT7 vector. The indicated plasmids were co-transformed into yeast strain Y2HGold. Transformants were selected for growth on -T-L medum. The colonies were then transferred to -T-L (left) and QDO+A+X (right) plates. (c) Co-immunoprecipitation between SLD5 and VSV-M-flag/HIV-M-flag. In total, 293 T cells were co-transfected with PCDH-SLD5 and VSV-M-flag/HIV-M-flag plasmids or the empty vector pcDNA3.0-flag. Then, 36 h post-transfection, the cells lysates were immunoprecipitated (IP) with anti-flag affinity gel and then subjected to immunoblotting (IB) with the indicated antibodies. (d) Co-immunoprecipitation between endogenous SLD5 and VSV-M-flag. Lysates of 293 T cells transfected with the pcDNA3.0-flag or pcDNA3.0-VSV-M-flag were IP with anti-flag affinity gel, followed by IB with the indicated antibodies. (e) Co-localization of SLD5 and VSV M. HeLa cells were co-transfected with pcDNA4.0-SLD5-HA and pcDNA3.0-VSV-M-flag plasmids, 24 h later, cells were fixed and stained with anti-flag and <t>anti-HA</t> antibodies, followed by fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated secondary antibodies, then analysed by confocal microscopy. Representative micrographs with scale bars representing 8 µm and 25 µm. (f) Unrooted phylogenetic tree of selected RNA viruses based on the M proteins. Complete M amino acid sequences were aligned by ClustalW using mega 7 and analysed by the maximum-likelihood method. The cladogram was generated using Fig Tree v1.4.4. Scales bars indicate amino acid substitutions per site. The tree is overlaid with coloured ellipses representing the various virus families or genera.

Goat Anti Ha Tag Polyclonal Antibody A00168 40, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-ha+antibody+a00168-40/goat+anti+ha/pmc08744269-231-8-16
Average 90 stars, based on 1 article reviews

goat anti-ha-tag polyclonal antibody a00168-40 - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Multiple RNA virus matrix proteins interact with SLD5 to manipulate host cell cycle"

Article Title: Multiple RNA virus matrix proteins interact with SLD5 to manipulate host cell cycle

Journal: The Journal of General Virology

doi: 10.1099/jgv.0.001697

Figure Legend Snippet: SLD5 interacts with matrix proteins of several RNA viruses. (a) The nuclear location sequences of M proteins of different RNA viruses were listed. Critical amino acids contributing to a NLS motif are coloured red. (b) Interaction between SLD5 and M proteins by the yeast two-hybrid assay. SLD5 was fused to the pGADT7 vector, M from ICV, MeV, SeV, VSV, ZEBOV, HIV and EIAV were fused to the pGBKT7 vector. The indicated plasmids were co-transformed into yeast strain Y2HGold. Transformants were selected for growth on -T-L medum. The colonies were then transferred to -T-L (left) and QDO+A+X (right) plates. (c) Co-immunoprecipitation between SLD5 and VSV-M-flag/HIV-M-flag. In total, 293 T cells were co-transfected with PCDH-SLD5 and VSV-M-flag/HIV-M-flag plasmids or the empty vector pcDNA3.0-flag. Then, 36 h post-transfection, the cells lysates were immunoprecipitated (IP) with anti-flag affinity gel and then subjected to immunoblotting (IB) with the indicated antibodies. (d) Co-immunoprecipitation between endogenous SLD5 and VSV-M-flag. Lysates of 293 T cells transfected with the pcDNA3.0-flag or pcDNA3.0-VSV-M-flag were IP with anti-flag affinity gel, followed by IB with the indicated antibodies. (e) Co-localization of SLD5 and VSV M. HeLa cells were co-transfected with pcDNA4.0-SLD5-HA and pcDNA3.0-VSV-M-flag plasmids, 24 h later, cells were fixed and stained with anti-flag and anti-HA antibodies, followed by fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated secondary antibodies, then analysed by confocal microscopy. Representative micrographs with scale bars representing 8 µm and 25 µm. (f) Unrooted phylogenetic tree of selected RNA viruses based on the M proteins. Complete M amino acid sequences were aligned by ClustalW using mega 7 and analysed by the maximum-likelihood method. The cladogram was generated using Fig Tree v1.4.4. Scales bars indicate amino acid substitutions per site. The tree is overlaid with coloured ellipses representing the various virus families or genera.

Techniques Used: Y2H Assay, Plasmid Preparation, Transformation Assay, Immunoprecipitation, Transfection, Western Blot, Staining, Confocal Microscopy, Generated, Virus

Expressing:

Article Title: SOX4 can redirect TGF-β-mediated SMAD3-transcriptional output in a context-dependent manner to promote tumorigenesis
Article Snippet: Protein expression of whole cell lysates was assessed by using the following primary antibodies: anti-FLAG (Sigma, F7425) and anti-HA (A00168-40, Genscript).

Immunoprecipitation:

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Journal: The Journal of General Virology

Article Title: Multiple RNA virus matrix proteins interact with SLD5 to manipulate host cell cycle

doi: 10.1099/jgv.0.001697

Figure Lengend Snippet: SLD5 interacts with matrix proteins of several RNA viruses. (a) The nuclear location sequences of M proteins of different RNA viruses were listed. Critical amino acids contributing to a NLS motif are coloured red. (b) Interaction between SLD5 and M proteins by the yeast two-hybrid assay. SLD5 was fused to the pGADT7 vector, M from ICV, MeV, SeV, VSV, ZEBOV, HIV and EIAV were fused to the pGBKT7 vector. The indicated plasmids were co-transformed into yeast strain Y2HGold. Transformants were selected for growth on -T-L medum. The colonies were then transferred to -T-L (left) and QDO+A+X (right) plates. (c) Co-immunoprecipitation between SLD5 and VSV-M-flag/HIV-M-flag. In total, 293 T cells were co-transfected with PCDH-SLD5 and VSV-M-flag/HIV-M-flag plasmids or the empty vector pcDNA3.0-flag. Then, 36 h post-transfection, the cells lysates were immunoprecipitated (IP) with anti-flag affinity gel and then subjected to immunoblotting (IB) with the indicated antibodies. (d) Co-immunoprecipitation between endogenous SLD5 and VSV-M-flag. Lysates of 293 T cells transfected with the pcDNA3.0-flag or pcDNA3.0-VSV-M-flag were IP with anti-flag affinity gel, followed by IB with the indicated antibodies. (e) Co-localization of SLD5 and VSV M. HeLa cells were co-transfected with pcDNA4.0-SLD5-HA and pcDNA3.0-VSV-M-flag plasmids, 24 h later, cells were fixed and stained with anti-flag and anti-HA antibodies, followed by fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated secondary antibodies, then analysed by confocal microscopy. Representative micrographs with scale bars representing 8 µm and 25 µm. (f) Unrooted phylogenetic tree of selected RNA viruses based on the M proteins. Complete M amino acid sequences were aligned by ClustalW using mega 7 and analysed by the maximum-likelihood method. The cladogram was generated using Fig Tree v1.4.4. Scales bars indicate amino acid substitutions per site. The tree is overlaid with coloured ellipses representing the various virus families or genera.

Article Snippet: Mouse anti-flag mAb (F1804) was purchased from Sigma; goat anti-HA-tag polyclonal antibody (A00168-40) was purchased from GenScript; mouse anti-β-actin (sc-47778) mAb was purchased from Santa Cruz Biotechnology; rabbit anti-phospho-IRF3 mAb (Ser386, ab76493), rabbit anti-phospho-TBK1 mAb (Ser172, ab109272) and rabbit anti-MX1 (ab95926) Abs were purchased from Abcam.

Techniques: Y2H Assay, Plasmid Preparation, Transformation Assay, Immunoprecipitation, Transfection, Western Blot, Staining, Confocal Microscopy, Generated, Virus

Journal: eLife

Article Title: MARCH8 inhibits viral infection by two different mechanisms

doi: 10.7554/eLife.57763

Figure Lengend Snippet:

Article Snippet: The fixed cells were incubated with primary antibodies, anti-T7 epitope mouse monoclonal antibody (Novagen, 69522–4) and anti-HA goat polyclonal antibody (GenScript, A00168-40).

Techniques: Recombinant, Sequencing, Transfection, Enzyme-linked Immunosorbent Assay, Luciferase, Immunoprecipitation, Protease Inhibitor, Western Blot, Immunofluorescence, Software